Controlling Culex Quinquefasciatus Say, 1823 (Diptera: Culicidae) Using Several Lysinibacillus Sphaericus Isolates Endogenic to Indonesia

Mosquito-borne diseases include tropical such as malaria, filariasis, dengue fever, chikungunya, yellow fever and cerebral are still major health problems in Indonesia on a global scale. Various methods have been used to overcome this, including controlling vector mosquitoes using the entomopathogenic microbial Lysinibacillus sphaericus. This study aims identify bacterial isolates collected based 16S rRNA gene carry out pathogenicity test of Cx. quinquefasciatus larvae. Bacterial this were from root soil, bird droppings guano. The identification type bacteria was carried fragment. Based results RNA sequence analysis 229C, 6B4, 6.2 4D21, it found that four included L. sphaericus species with similarity scores ranging 97% 100%. measured mortality larvae know whether has equal or higher than strain 1593. 6 15.4, 1593, 4D21 showed isolate 15.4 highest larval rate, so is potentially biological agent control disease mosquitoes.